A Colorimetric Method for the Determination of Serum Arginase Activity
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چکیده
منابع مشابه
Colorimetric method for rapid determination of serum arginase.
A method for the determination of serum arginase is given which combines the enzymatic formation of urea with the sensitive method of Coulombe (1) for measuring this substance. This procedure allows more accurate determinations in the normal range than do previous methods described and is convenient for clinical routine. Significant difference is found between the mean values of normal men and ...
متن کاملColorimetric determination of blood arginase.
N EXTENSIVE LITERATURE Ofl arginase activity in tissues and tissue homogenates has accumulated in recent years. Roberts (1) has studied the arginase activity of liver, kidney, and epidermis of the Swiss mouse and of a transplantable squamous cell carcinoma. He found that liver had the greatest activity, followed by carcinoma, epidermis, and kidney. Sano (2) isolated an enzyme from rabbit intest...
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HAVE INSTITUTED a colorimetric chloride procedure which has been in use for 3 years in our laboratory and has met with great success. This colorimetric method is based on a procedure used in automated syStems* (2, 3). It is extremely simple, quite accurate, and gives highly reproducible results even in the hands of student technicians. Those laboratories not having access to a chloridometer or ...
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A colorimetric method for the determination of serum guanase has been developed whereby ammonia, formed by the deamination of guanine, is measured by the phenatehypochlorite reaction. Variations in substrate concentration, time of incubation, pH, temperature, and concentration of enzyme have been investigated with respect to enzyme activity. The determination of serum guanase appears to be usef...
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An accurate, simple colorimetric method for the determination of serum alpha-hydroxybutyric dehydrogenase activity is described. The method is suitable for routine laboratory use.
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1969
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/15.2.162